How to Study Protein-protein Interactions
نویسندگان
چکیده
منابع مشابه
Discovering Domains Mediating Protein Interactions
Background: Protein-protein interactions do not provide any direct information regarding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting domain pairs. However they do not consider the in...
متن کاملHow Complex are Complex Protein-protein Interactions?
The extraction of protein-protein interactions (PPI) from text requires a formal PPI representation. We use the BioInfer and GENIA corpora to study two such representations: a “binary” interaction model consisting of pairs of proteins and a “complex” model where interactions are defined as a network of proteins and their relations. As both of these formats can be seen as graphs, we contrast the...
متن کاملBiotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Here we present a protocol to study intracellular protein-protein interactions that is based on the widely used biotin-avidin pull-down system. The modification presented includes the combination of this technique with cell-penetrating sequences. We propose to design cell-penetrating baits that can be incubated with living cells instead of cell lysates and therefore the interactions found will ...
متن کاملHow Many Protein-Protein Interactions Types Exist in Nature?
"Protein quaternary structure universe" refers to the ensemble of all protein-protein complexes across all organisms in nature. The number of quaternary folds thus corresponds to the number of ways proteins physically interact with other proteins. This study focuses on answering two basic questions: Whether the number of protein-protein interactions is limited and, if yes, how many different qu...
متن کاملHow Structure Defines Affinity in Protein-Protein Interactions
Protein-protein interactions (PPI) in nature are conveyed by a multitude of binding modes involving various surfaces, secondary structure elements and intermolecular interactions. This diversity results in PPI binding affinities that span more than nine orders of magnitude. Several early studies attempted to correlate PPI binding affinities to various structure-derived features with limited suc...
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ژورنال
عنوان ژورنال: Acta Chimica Slovenica
سال: 2016
ISSN: 1318-0207
DOI: 10.17344/acsi.2016.2419